Process Parameters
Operating Ranges by Process Zone
Process ZoneTarget DO (mg/L)Notes
Aeration Basin (Nitrification)2.0 – 4.0Minimum 2.0 for full nitrification
Aeration Basin (General)1.5 – 3.0Below 1.0 risks filamentous growth
Anoxic Zone (Denitrification)< 0.2DO inhibits denitrification
Anaerobic Zone (EBPR)< 0.05Critical for phosphorus release
Secondary Clarifier Effluent4.0 – 8.0Higher DO indicates good aeration
Digester (Anaerobic)0Strict anaerobic conditions required
Operating Ranges by Process
ProcesspH RangeNotes
Activated Sludge6.5 – 8.5Optimal range for biological activity
Nitrification7.2 – 8.0Below 6.8 significantly inhibits nitrifiers
Denitrification6.5 – 8.0Less sensitive to pH than nitrification
Anaerobic Digestion6.8 – 7.4Below 6.5 = acid phase; above 8.0 = inhibited
Chemical Phosphorus Removal5.5 – 7.0Alum optimal around 6.0–7.0
Effluent Discharge (typical)6.0 – 9.0Varies by permit
Design & Operational Targets
ParameterTypical RangeUnits
F/M Ratio (Conventional AS)0.2 – 0.4lbs BOD / lb MLVSS / day
F/M Ratio (Extended Aeration)0.04 – 0.1lbs BOD / lb MLVSS / day
MCRT (Nitrification)10 – 20days (varies with temp)
MCRT (Conventional)5 – 15days
SVI (Good Settling)80 – 150mL/g
SVI (Bulking)> 200mL/g
Surface Loading Rate (Clarifier)400 – 800gpd/ft²
Solids Loading Rate (Clarifier)20 – 30lbs/day/ft²
VS Loading Rate (Digester)0.1 – 0.3lbs VS/day/ft³
Digester Detention Time15 – 30days
VSR (Anaerobic Digestion)38 – 55% (Class B minimum 38%)
Biological Process Performance by Temperature
TemperatureDO SaturationNitrificationNotes / Guidance
< 5°C / < 41°F≥ 12.8 mg/LSeverely inhibited — < 30% of peak rateSRT > 25 days; nitrification may fail; digestion near-zero
5–10°C / 41–50°F11.3 – 12.4 mg/LSignificantly reduced — ~30–50% of peak rateIncrease SRT to 15–25 days; monitor effluent ammonia closely
10–15°C / 50–59°F10.1 – 11.3 mg/LReduced — ~50–75% of peak rateSRT 12–18 days; watch for seasonal compliance issues
15–20°C / 59–68°F9.1 – 10.1 mg/LNear-normalStandard temperate operating range; standard SRT adequate
20–25°C / 68–77°F8.2 – 9.1 mg/LOptimalStandard design condition; high biological activity
> 25°C / > 77°F< 8.2 mg/LGood, but O₂ demand risesMonitor blower capacity; lower DO saturation tightens margins
35–37°C / 95–99°F6.7 – 6.8 mg/LN/A — digester rangeOptimal mesophilic anaerobic digestion; target for heated digesters
55°C / 131°F~5.5 mg/LN/A — digester rangeOptimal thermophilic digestion; PFRP / Class A treatment path
Common Wastewater Conversions
FromToFactor
MGDGPM× 694.44
MGDCFS× 1.5472
MGDL/s× 43.813
MGD × mg/Llbs/day× 8.34
mg/Llbs/MG× 8.34
ft³gallons× 7.481
acresft²× 43,560
°F°C(°F − 32) × 5/9
Chemistry & Dosing
Targets & Nitrification Consumption
Parameter / ProcessTarget RangeNotes
Aeration Basin Alkalinity80 – 120 mg/L as CaCO₃Below 80: pH drops, nitrification destabilizes
Effluent Alkalinity (minimum)≥ 50 mg/L as CaCO₃Residual alkalinity after nitrification; below this risks pH crash
Nitrification consumption7.14 mg alk / mg NH₄⁺-NTheoretical; actual 6.0–7.0 in practice
Denitrification recovery3.57 mg alk / mg NO₃⁻-NDenitrification returns ~50% of nitrification alkalinity draw
Sodium bicarbonate addition84 g/mol1 lb NaHCO₃ adds 0.595 lbs alkalinity as CaCO₃
Hydrated lime addition74 g/mol Ca(OH)₂More reactive than NaHCO₃; raises pH quickly — dose carefully
pH below which to supplement< 6.8 in basinSupplement alkalinity; don't wait for effluent pH to drop
O₂ Requirements & Transfer Factors
ParameterValueNotes
BOD removal O₂ demand (theoretical)1.0 lb O₂ / lb BOD₅Actual 0.8–1.2 depending on SRT and endogenous demand
Nitrification O₂ demand4.57 lb O₂ / lb NH₄⁺-NDoubles total aeration load at high ammonia influent
Denitrification O₂ credit2.86 lb O₂ / lb NO₃⁻-NReduces net aeration demand; credit for anoxic zone
Endogenous respiration0.08 – 0.12 lb O₂ / lb VSS / dayAdd to BOD demand for total basin O₂ requirement
Alpha factor (fine bubble, MLSS)0.65 – 0.85Reduces clean-water SOTR in mixed liquor; use 0.70–0.75 for design
Beta factor0.95 – 0.98TDS/salinity correction on DO saturation; near 1.0 for municipal WW
Fine bubble diffuser SOTE20 – 35%Per meter submergence; decreases with diffuser fouling
Coarse bubble SOTE8 – 15%Lower efficiency; simpler maintenance; used for mixing
Typical Doses & Application Points
ChemicalTypical DoseApplication / Notes
Alum (liquid, 48%)5 – 15 mg/L as AlPhosphorus precipitation; optimal pH 6.0–7.0; reduces alkalinity
Ferric chloride (liquid, 37–42%)5 – 20 mg/L as FePhosphorus precipitation; effective pH 4.5–8.0; more corrosive than alum
Sodium hypochlorite (12.5%)2 – 10 mg/L as Cl₂Disinfection; actual dose depends on CT requirement and effluent demand
Polymer (cationic, belt press)10 – 25 lbs/dry tonBiosolids conditioning; jar-test to optimize; higher dose ≠ better cake
Polymer (cationic, GBT)5 – 15 lbs/dry tonLower dose than belt press; inject close to static mixer
Polymer (anionic, DAF)2 – 8 lbs/dry tonDAF thickening; charge must match sludge — jar-test required
Sodium bisulfite (dechlorination)1 mg/L per 1 mg/L Cl₂Stoichiometric; slight overdose acceptable; monitor residual
Caustic soda (50% NaOH)Varies — pH controlledpH adjustment; extremely reactive — use automated dosing with pH feedback
Solids & Biosolids
Typical % Solids by Sludge Type
Sludge TypeTypical % TSNotes
Raw primary sludge4 – 6%Higher with longer primary HRT
Waste activated sludge (WAS)0.5 – 1.5%Dilute; thickening required before digestion
Primary + WAS blend2 – 4%Blend ratio determines concentration
Aerobically digested sludge1 – 3%Often thinner than anaerobically digested
Anaerobically digested (liquid)2 – 4%Reduced VSS; stabilized
GBT thickened WAS4 – 7%With polymer conditioning
Belt press dewatered cake15 – 25%Varies by feed solids and polymer dose
Centrifuge dewatered cake18 – 28%More consistent than belt press
Heat dried biosolids90 – 95%Pellet or granule form; Class A typical
Class A vs. Class B — Federal 503 Standards
CriterionClass AClass B
Pathogen standardFecal coliform < 1,000 MPN/g TS or Salmonella < 3 MPN/4g TSFecal coliform < 2,000,000 MPN/g TS (geometric mean)
Vector attraction reduction (VAR)One of Options 1–8 requiredOne of Options 1–10 required
VSR (anaerobic digestion)≥ 38% + time/temp requirement≥ 38% (Van Kleeck formula)
Common VAR method (anaerobic)PFRP: thermophilic digestion (55°C / 15+ days)PSRP: mesophilic digestion (35–55°C / 15+ days HRT)
Land application restrictionsNo site access restrictions requiredSite access restrictions: 30-day crop harvest hold, grazing restrictions
Public contact sitesAllowed without restrictions12-month site access restriction
Common treatment pathsThermophilic digestion, heat drying, alkaline stabilization (≥ 70°C)Mesophilic anaerobic digestion, aerobic digestion, composting
Testing frequencyMore frequent — verify each pathogen batchLess frequent once process reliability established
Laboratory
Common Causes, Symptoms & Control Responses
OrganismAssociated ConditionsSymptomsFirst Response
Nocardia spp.Long SRT; high FOG loading; warm tempsStable brown/tan foam; chocolate mousse appearance on basin surfaceReduce SRT; chlorinate foam; address upstream FOG sources
Microthrix parvicellaLong SRT; low temp (< 15°C); fat/oil loadingBrown stable foam; bulking; worsens in winterReduce SRT; foam wasting; control fat and oil loading
Type 021NSulfide in feed; low DO; low F/MTight non-settling floc; high SVI; pin floc possibleIncrease DO; eliminate septic conditions; pre-aerate force main
Thiothrix spp.Sulfide or reduced sulfur in influent; low DOBulking; rosette structures visible under microscopeEliminate H₂S sources; increase DO; pre-aerate force main
Type 0041 / 0675Very low F/M; nutrient deficiency (N or P)Viscous / zoogloeal bulking; stringy or gel-like flocIncrease F/M; verify N:P:BOD ratio; check influent nutrient loading
Type 1701Low DO; high soluble substrateDiffuse floc growth; poor settlingIncrease DO setpoint; check for anoxic zone bleed-over
BeggiatoaHigh sulfide; anaerobic conditions in basinWhite surface mat; settling interferenceImprove aeration uniformity; address septicity in influent
Standard Methods Reference — Preservation & Hold Times
ParameterStandard MethodPreservation & Hold TimeNotes
BOD₅SM 5210BCool 4°C; no acid; 48-hour holdSeed per method; dilute to keep depletion 2–8 mg/L DO
TSSSM 2540DCool 4°C; 7-day holdGlass fiber filter (GF/C); filter within 24h preferred
VSSSM 2540ECool 4°C; 7-day holdSequential with TSS using same filter; pre-ignite filters
Total AlkalinitySM 2320BCool 4°C; 14-day holdDo not acidify; titrate within 15 min of collection when possible
pHSM 4500-H⁺ BMeasure immediately; no holdCalibrate with fresh buffers before each session; ATC required
Dissolved OxygenSM 4500-O GMeasure immediately; no holdMembrane or optical sensor; calibrate at start of each shift
Ammonia-NSM 4500-NH₃H₂SO₄ to pH < 2; cool 4°C; 28-day holdColorimetric or ISE; distill if matrix interference suspected
Nitrate-NSM 4500-NO₃⁻Cool 4°C; 48-hour holdIon chromatography or cadmium reduction; check chloride interference
Total PhosphorusSM 4500-P B/EH₂SO₄ to pH < 2; cool 4°C; 28-day holdDigest before colorimetric analysis
Total Kjeldahl NitrogenSM 4500-NH₂SO₄ to pH < 2; cool 4°C; 28-day holdOrganic-N + ammonia-N combined; requires digestion step
Fecal ColiformSM 9221E / 9222DNa₂S₂O₃; cool 4°C; 6-hour holdQuench chlorine at collection; strict hold time for permit samples
Chlorine ResidualSM 4500-ClMeasure immediately; no holdDPD colorimetric preferred; field measurement at discharge point